|
|
NAD+ + (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA <=> H+ + NADH + 2-methylacetoacetyl-CoA
|
1.1.1.178
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
1.1.1.35
3-hydroxyacyl-CoA dehydrogenase
|
–
–
|
| |
BRENDA:
BR29237;
BS187429
KEGG:
R04203
MetaCyc:
1.1.1.178-RXN
SABIO-RK:
3919
|
|
|
|
methylmalonate + H2O2 <=> L-2-methyl-3-oxopropionate + O2 + H2O
|
1.2.3.1
aldehyde oxidase
|
–
|
| |
KEGG:
R03871
|
|
|
|
enzyme_N6-(dihydrolipoyl)lysine + NAD+ <=> [dihydrolipoyllysine-residue(2-methylpropanoyl)transferase]-lipoyllysine + NADH + H+
|
1.8.1.4
dihydrolipoyl dehydrogenase
|
–
|
| |
BRENDA:
BR36970
KEGG:
R03815;
R07618;
R08550
MetaCyc:
1.8.1.4-RXN;
RXN-7716
SABIO-RK:
9140
|
|
|
|
(S)-3-methyl-2-oxopentanoate + thiamine_diphosphate <=> 2-Methyl-1-hydroxybutyl-ThPP + CO2
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07603
|
|
|
|
CoA + acetoacetate + ATP <=> acetoacetyl-CoA + diphosphate + AMP
|
6.2.1.16
acetoacetate---CoA ligase
|
–
|
| |
BRENDA:
BR67
KEGG:
R01357
MetaCyc:
ACETOACETATE--COA-LIGASE-RXN
SABIO-RK:
1561
|
|
|
|
(R)-2-methylmalonyl-CoA <=> succinyl-CoA
|
5.4.99.2
methylmalonyl-CoA mutase
5.4.99.63
ethylmalonyl-CoA mutase
5.4.99.64
2-hydroxyisobutanoyl-CoA mutase
|
–
–
–
|
| |
BRENDA:
BR249;
BS380077;
BS390473
KEGG:
R00833
MetaCyc:
METHYLMALONYL-COA-MUT-RXN
SABIO-RK:
10453;
1174
|
|
|
|
L-2-methyl-3-oxopropionate + NAD+ + H2O <=> methylmalonate + NADH + H+
|
1.2.1.3
aldehyde dehydrogenase (NAD+)
|
–
|
| |
KEGG:
R03869
|
|
|
|
(S)-3-hydroxy-3-methylglutaryl-CoA <=> acetoacetate + acetyl-CoA
|
4.1.3.4
hydroxymethylglutaryl-CoA lyase
|
–
|
| |
BRENDA:
BR395;
BS416230
KEGG:
R01360
MetaCyc:
HYDROXYMETHYLGLUTARYL-COA-LYASE-RXN
SABIO-RK:
1564
|
|
|
|
3-hydroxyisovaleryl-CoA <=> 3-Hydroxy-3-methylbutyrate
|
not assigned
|
–
|
| |
KEGG:
R10759
|
|
|
|
succinyl-CoA + acetoacetate <=> succinate + acetoacetyl-CoA
|
2.8.3.18
succinyl-CoA:acetate CoA-transferase
2.8.3.5
3-oxoacid CoA-transferase
|
–
–
|
| |
BRENDA:
BS360598
KEGG:
R00410
MetaCyc:
RXNI-2
SABIO-RK:
867
|
|
|
|
L-leucine + NAD+ + H2O <=> H+ + NH3 + NADH + 4-methyl-2-oxopentanoate
|
1.4.1.1
alanine dehydrogenase
1.4.1.20
phenylalanine dehydrogenase
1.4.1.23
valine dehydrogenase (NAD+)
1.4.1.8
valine dehydrogenase (NADP+)
1.4.1.9
leucine dehydrogenase
|
–
–
–
–
–
|
| |
BRENDA:
BR33798
KEGG:
R01088
MetaCyc:
LEUCINE-DEHYDROGENASE-RXN
SABIO-RK:
1350
|
|
|
|
acetoacetyl-CoA + H2O + acetyl-CoA <=> H+ + CoA + (S)-3-hydroxy-3-methylglutaryl-CoA
|
2.3.3.10
hydroxymethylglutaryl-CoA synthase
|
–
|
| |
BRENDA:
BR44119
KEGG:
R01978
MetaCyc:
HYDROXYMETHYLGLUTARYL-COA-SYNTHASE-RXN
SABIO-RK:
2021
|
|
|
|
3-Methyl-1-hydroxybutyl-ThPP + Enzyme_N6-(lipoyl)lysine <=> [dihydrolipoyllysine-residue_(2-methylpropanoyl)transferase]_S-(3-methylbutanoyl)dihydrolipoyllysine + thiamine_diphosphate
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07602
|
|
|
|
2-methylacetoacetyl-CoA + CoA <=> propionyl-CoA + acetyl-CoA
|
2.3.1.16
acetyl-CoA C-acyltransferase
2.3.1.9
acetyl-CoA C-acetyltransferase
|
–
–
|
| |
BRENDA:
BS407013
KEGG:
R00927
MetaCyc:
METHYLACETOACETYLCOATHIOL-RXN
|
|
|
|
L-leucine + 2-oxoglutarate <=> L-glutamate + 4-methyl-2-oxopentanoate
|
2.6.1.1
aspartate transaminase
2.6.1.2
alanine transaminase
2.6.1.39
2-aminoadipate transaminase
2.6.1.42
branched-chain-amino-acid transaminase
2.6.1.57
aromatic-amino-acid transaminase
2.6.1.6
leucine transaminase
2.6.1.67
2-aminohexanoate transaminase
2.6.1.9
histidinol-phosphate transaminase
|
–
–
–
–
–
–
–
–
|
| |
BRENDA:
BR47691
KEGG:
R01090
MetaCyc:
BRANCHED-CHAINAMINOTRANSFERLEU-RXN
SABIO-RK:
1352
|
|
|
|
L-valine + 2-oxoglutarate <=> L-glutamate + 2-oxoisovalerate
|
2.6.1.39
2-aminoadipate transaminase
2.6.1.42
branched-chain-amino-acid transaminase
2.6.1.6
leucine transaminase
2.6.1.66
valine---pyruvate transaminase
|
–
–
–
–
|
| |
BRENDA:
BS356553
KEGG:
R01214
MetaCyc:
BRANCHED-CHAINAMINOTRANSFERVAL-RXN
SABIO-RK:
1446
|
|
|
|
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA <=> tigloyl-CoA + H2O
|
4.2.1.17
enoyl-CoA hydratase
|
–
|
| |
BRENDA:
BS444413
KEGG:
R04204
MetaCyc:
TIGLYLCOA-HYDROXY-RXN
SABIO-RK:
3920
|
|
|
|
L-isoleucine + 2-oxoglutarate <=> L-glutamate + (S)-3-methyl-2-oxopentanoate
|
2.6.1.42
branched-chain-amino-acid transaminase
2.6.1.67
2-aminohexanoate transaminase
|
–
–
|
| |
BRENDA:
BS356522;
BS452227
KEGG:
R02199
MetaCyc:
BRANCHED-CHAINAMINOTRANSFERILEU-RXN
SABIO-RK:
2171;
9086
|
|
|
|
(S)-2-methylbutanoyl-CoA + enzyme_N6-(dihydrolipoyl)lysine <=> CoA + [dihydrolipoyllysine-residue_(2-methylpropanoyl)transferase]_S-(2-methylbutanoyl)dihydrolipoyllysine
|
2.3.1.168
dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
|
–
|
| |
KEGG:
R03174
|
|
|
|
H2O + (S)-methylmalonyl-CoA <=> H+ + CoA + methylmalonate
|
3.1.2.17
(S)-methylmalonyl-CoA hydrolase
3.1.2.20
acyl-CoA hydrolase
3.1.2.4
3-hydroxyisobutyryl-CoA hydrolase
|
–
–
–
|
| |
BRENDA:
BR47932;
BS401387
KEGG:
R02764
MetaCyc:
S-METHYLMALONYL-COA-HYDROLASE-RXN
SABIO-RK:
11293;
2641
|
|
|
|
3-hydroxyisovaleryl-CoA <=> 3-methylcrotonoyl-CoA + H2O
|
4.2.1.17
enoyl-CoA hydratase
|
–
|
| |
KEGG:
R04137
SABIO-RK:
3858
|
|
|
|
4-methyl-2-oxopentanoate + thiamine_diphosphate <=> 3-Methyl-1-hydroxybutyl-ThPP + CO2
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07601
|
|
|
|
2-Methyl-1-hydroxypropyl-ThPP + Enzyme_N6-(lipoyl)lysine <=> [dihydrolipoyllysine-residue_(2-methylpropanoyl)transferase]_S-(2-methylpropanoyl)dihydrolipoyllysine + thiamine_diphosphate
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07600
|
|
|
|
2-Methyl-1-hydroxybutyl-ThPP + Enzyme_N6-(lipoyl)lysine <=> [dihydrolipoyllysine-residue_(2-methylpropanoyl)transferase]_S-(2-methylbutanoyl)dihydrolipoyllysine + thiamine_diphosphate
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07604
|
|
|
|
(S)-2-methylbutanoyl-CoA + acceptor <=> tigloyl-CoA + reduced_acceptor
|
1.3.8.1
short-chain acyl-CoA dehydrogenase
1.3.8.7
medium-chain acyl-CoA dehydrogenase
1.3.99.12
2-methylacyl-CoA dehydrogenase
|
–
–
–
|
| |
BRENDA:
BS258775
KEGG:
R03172
|
|
|
|
2-oxoisovalerate + CoA + oxidized_ferredoxin <=> isobutyryl-CoA + CO2 + reduced_ferredoxin_[iron-sulfur]_cluster + H+
|
1.2.7.7
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin)
|
–
|
| |
BRENDA:
BR48034
KEGG:
R07160
MetaCyc:
1.2.7.7-RXN
|
|
|
|
isobutyryl-CoA + acceptor <=> methacryloyl-CoA + reduced_acceptor
|
1.3.8.1
short-chain acyl-CoA dehydrogenase
1.3.8.7
medium-chain acyl-CoA dehydrogenase
1.3.99
1.3.99.12
2-methylacyl-CoA dehydrogenase
|
–
–
–
–
|
| |
BRENDA:
BS258777
KEGG:
R02661
|
|
|
|
3-methylcrotonoyl-CoA + HCO3- + ATP <=> trans-3-methylglutaconyl-CoA + ADP + phosphate + H+
|
6.4.1.4
methylcrotonoyl-CoA carboxylase
6.4.1.5
geranoyl-CoA carboxylase
|
–
–
|
| |
BRENDA:
BR156
KEGG:
R04138
MetaCyc:
METHYLCROTONYL-COA-CARBOXYLASE-RXN
SABIO-RK:
3859
|
|
|
|
(S)-3-methyl-2-oxopentanoate + CoA + oxidized_ferredoxin <=> (S)-2-methylbutanoyl-CoA + CO2 + reduced_ferredoxin_[iron-sulfur]_cluster + H+
|
1.2.7.7
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin)
|
–
|
| |
KEGG:
R08567
MetaCyc:
RXN-19503
|
|
|
|
(S)-3-hydroxy-2-methylpropanoyl-CoA <=> methacryloyl-CoA + H2O
|
4.2.1.17
enoyl-CoA hydratase
|
–
|
| |
KEGG:
R04224
MetaCyc:
METHYLACYLYLCOA-HYDROXY-RXN
SABIO-RK:
3935
|
|
|
|
L-3-hydroxyisobutyrate + NAD+ <=> H+ + NADH + L-2-methyl-3-oxopropionate
|
1.1.1.291
2-hydroxymethylglutarate dehydrogenase
1.1.1.31
3-hydroxyisobutyrate dehydrogenase
1.1.1.35
3-hydroxyacyl-CoA dehydrogenase
|
–
–
–
|
| |
BRENDA:
BS188039
KEGG:
R05066
MetaCyc:
3-HYDROXYISOBUTYRATE-DEHYDROGENASE-RXN
SABIO-RK:
4693
|
|
|
|
(S)-3-hydroxy-2-methylpropanoyl-CoA + H2O <=> L-3-hydroxyisobutyrate + H+ + CoA
|
3.1.2.4
3-hydroxyisobutyryl-CoA hydrolase
|
–
|
| |
BRENDA:
BS364470;
BS446008
KEGG:
R05064
MetaCyc:
3-HYDROXYISOBUTYRYL-COA-HYDROLASE-RXN
SABIO-RK:
4692
|
|
|
|
isovaleryl-CoA + enzyme_N6-(dihydrolipoyl)lysine <=> CoA + [dihydrolipoyllysine-residue_(2-methylpropanoyl)transferase]_S-(3-methylbutanoyl)dihydrolipoyllysine
|
2.3.1.168
dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
|
–
|
| |
KEGG:
R04097
|
|
|
|
acetyl-CoA <=> acetoacetyl-CoA + CoA
|
1.1.1.34
hydroxymethylglutaryl-CoA reductase (NADPH)
2.3.1.155
acetyl-CoA C-myristoyltransferase
2.3.1.16
acetyl-CoA C-acyltransferase
2.3.1.38
[acyl-carrier-protein] S-acetyltransferase
2.3.1.9
acetyl-CoA C-acetyltransferase
|
–
–
–
–
–
|
| |
BRENDA:
BR44092
KEGG:
R00238
MetaCyc:
ACETYL-COA-ACETYLTRANSFER-RXN
SABIO-RK:
531
|
|
|
|
isovaleryl-CoA <=> Branched_chain_fatty_acid
|
not assigned
|
–
|
| |
KEGG:
R04098
|
|
|
|
L-2-methyl-3-oxopropionate + L-alanine <=> (S)-3-amino-2-methylpropanoate + pyruvate
|
2.6.1
2.6.1.18
beta-alanine---pyruvate transaminase
|
–
–
|
| |
KEGG:
R04187
MetaCyc:
RXN-14265
|
|
|
|
methylmalonate + CoA + ATP <=> (R)-2-methylmalonyl-CoA + AMP + diphosphate
|
6.2.1
|
–
|
| |
KEGG:
R03383
|
|
|
|
(R)-2-methylmalonyl-CoA <=> (S)-methylmalonyl-CoA
|
5.1.99.1
methylmalonyl-CoA epimerase
|
–
|
| |
BRENDA:
BR204;
BS970
KEGG:
R02765
MetaCyc:
METHYLMALONYL-COA-EPIM-RXN
SABIO-RK:
2642
|
|
|
|
L-isoleucine + H2O + O2 <=> (S)-3-methyl-2-oxopentanoate + NH3 + H2O2
|
1.4.3.2
L-amino-acid oxidase
|
–
|
| |
KEGG:
R02197
|
|
|
|
4-methyl-2-oxopentanoate + CoA + oxidized_ferredoxin <=> isovaleryl-CoA + CO2 + reduced_ferredoxin_[iron-sulfur]_cluster + H+
|
1.2.7.7
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin)
1.2.7.8
indolepyruvate ferredoxin oxidoreductase
|
–
–
|
| |
BRENDA:
BS359415
KEGG:
R08566
MetaCyc:
RXN-19534
|
|
|
|
(S)-3-hydroxy-3-methylglutaryl-CoA <=> H2O + trans-3-methylglutaconyl-CoA
|
4.2.1.18
methylglutaconyl-CoA hydratase
|
–
|
| |
BRENDA:
BR3388
KEGG:
R02085
MetaCyc:
METHYLGLUTACONYL-COA-HYDRATASE-RXN
SABIO-RK:
2095
|
|
|
|
2-Methyl-3-oxopropanoate + CoA + NAD+ <=> propionyl-CoA + CO2 + NADH
|
1.2.1.18
malonate-semialdehyde dehydrogenase (acetylating)
1.2.1.27
methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
|
–
–
|
| |
BRENDA:
BS189112
KEGG:
R00922
SABIO-RK:
1242
|
|
|
|
2-oxoisovalerate + thiamine_diphosphate <=> 2-Methyl-1-hydroxypropyl-ThPP + CO2
|
1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)
|
–
|
| |
KEGG:
R07599
|
|
|
|
L-valine + H2O + NAD+ <=> 2-oxoisovalerate + NH3 + NADH + H+
|
1.4.1.1
alanine dehydrogenase
1.4.1.2
glutamate dehydrogenase
1.4.1.20
phenylalanine dehydrogenase
1.4.1.23
valine dehydrogenase (NAD+)
1.4.1.5
L-amino-acid dehydrogenase
1.4.1.8
valine dehydrogenase (NADP+)
1.4.1.9
leucine dehydrogenase
|
–
–
–
–
–
–
–
|
| |
BRENDA:
BR50099;
BS408366;
BS451572
KEGG:
R01434
MetaCyc:
RXN-13184
SABIO-RK:
1619
|
|
|
|
isobutyryl-CoA <=> Branched_chain_fatty_acid
|
not assigned
|
–
|
| |
KEGG:
R02663
|
|
|
|
isovaleryl-CoA + FAD <=> 3-methylcrotonoyl-CoA + FADH2
|
1.3.8.4
isovaleryl-CoA dehydrogenase
1.3.8.7
medium-chain acyl-CoA dehydrogenase
|
–
–
|
| |
BRENDA:
BS424839
KEGG:
R04095
SABIO-RK:
3822
|
|
|
|
(R)-3-Amino-2-methylpropanoate + pyruvate <=> 2-Methyl-3-oxopropanoate + L-alanine
|
2.6.1.40
(R)-3-amino-2-methylpropionate---pyruvate transaminase
|
–
|
| |
BRENDA:
BR47992
KEGG:
R02050
|
|
|
|
ATP + propionyl-CoA + HCO3- <=> H+ + (S)-methylmalonyl-CoA + phosphate + ADP
|
6.4.1.2
acetyl-CoA carboxylase
6.4.1.3
propionyl-CoA carboxylase
|
–
–
|
| |
BRENDA:
BR155
KEGG:
R01859
MetaCyc:
PROPIONYL-COA-CARBOXY-RXN
SABIO-RK:
1929
|
|
|
|
2-oxoglutarate + (S)-3-amino-2-methylpropanoate <=> L-glutamate + L-2-methyl-3-oxopropionate
|
2.6.1.22
(S)-3-amino-2-methylpropionate transaminase
|
–
|
| |
KEGG:
R04188
MetaCyc:
2.6.1.22-RXN
SABIO-RK:
3906
|
|
|
|
isobutyryl-CoA + enzyme_N6-(dihydrolipoyl)lysine <=> CoA + enzyme_N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
|
2.3.1.168
dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
|
–
|
| |
BRENDA:
BR48046
KEGG:
R02662
|
|
|
|
(S)-2-methylbutanoyl-CoA <=> Branched_chain_fatty_acid
|
not assigned
|
–
|
| |
KEGG:
R03175
|
|
