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Valine, leucine and isoleucine biosynthesis

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
2-isopropylmaleate + H2O <=> (2S)-2-isopropylmalate 4.2.1.33 3-isopropylmalate dehydratase

2-isopropylmaleate + H2O <=> (2S)-2-isopropylmalate 4.2.1.33 3-isopropylmalate dehydratase

L-leucine + NAD+ + H2O <=> H+ + NH3 + NADH + 4-methyl-2-oxopentanoate 1.4.1.1 alanine dehydrogenase
1.4.1.20 phenylalanine dehydrogenase
1.4.1.23 valine dehydrogenase (NAD+)
1.4.1.8 valine dehydrogenase (NADP+)
1.4.1.9 leucine dehydrogenase





L-isoleucine + H2O + NAD+ <=> NH3 + (S)-3-methyl-2-oxopentanoate + NADH + H+ 1.4.1.1 alanine dehydrogenase
1.4.1.2 glutamate dehydrogenase
1.4.1.20 phenylalanine dehydrogenase
1.4.1.23 valine dehydrogenase (NAD+)
1.4.1.5 L-amino-acid dehydrogenase
1.4.1.8 valine dehydrogenase (NADP+)
1.4.1.9 leucine dehydrogenase







(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ <=> 3-hydroxy-3-methyl-2-oxobutanoic_acid + NADPH + H+ 1.1.1.86 ketol-acid reductoisomerase (NADP+)

(S)-2-hydroxy-2-ethyl-3-oxobutanoate <=> (R)-3-hydroxy-3-methyl-2-oxopentanoate 1.1.1.86 ketol-acid reductoisomerase (NADP+)
5.4.99.3 2-acetolactate mutase


L-threonine <=> 2-oxobutyrate + NH3 4.2.3.1 threonine synthase
4.3.1.17 L-serine ammonia-lyase
4.3.1.19 threonine ammonia-lyase



2-oxoisovalerate + acetyl-CoA + H2O <=> H+ + (2S)-2-isopropylmalate + CoA 2.3.3.13 2-isopropylmalate synthase
2.3.3.17 methylthioalkylmalate synthase


L-leucine + 2-oxoglutarate <=> L-glutamate + 4-methyl-2-oxopentanoate 2.6.1.1 aspartate transaminase
2.6.1.2 alanine transaminase
2.6.1.39 2-aminoadipate transaminase
2.6.1.42 branched-chain-amino-acid transaminase
2.6.1.57 aromatic-amino-acid transaminase
2.6.1.6 leucine transaminase
2.6.1.67 2-aminohexanoate transaminase
2.6.1.9 histidinol-phosphate transaminase








pyruvate + L-valine <=> L-alanine + 2-oxoisovalerate 2.6.1.12 alanine---oxo-acid transaminase
2.6.1.42 branched-chain-amino-acid transaminase
2.6.1.51 serine---pyruvate transaminase
2.6.1.66 valine---pyruvate transaminase




(2R,3S)-3-isopropylmalate + NAD+ <=> (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ 1.1.1.85 3-isopropylmalate dehydrogenase

L-valine + 2-oxoglutarate <=> L-glutamate + 2-oxoisovalerate 2.6.1.39 2-aminoadipate transaminase
2.6.1.42 branched-chain-amino-acid transaminase
2.6.1.6 leucine transaminase
2.6.1.66 valine---pyruvate transaminase




(R)-2,3-dihydroxy-3-methylbutanoate <=> 2-oxoisovalerate + H2O 4.2.1.9 dihydroxy-acid dehydratase

L-isoleucine + 2-oxoglutarate <=> L-glutamate + (S)-3-methyl-2-oxopentanoate 2.6.1.42 branched-chain-amino-acid transaminase
2.6.1.67 2-aminohexanoate transaminase


H+ + (2S)-2-isopropyl-3-oxosuccinate <=> 4-methyl-2-oxopentanoate + CO2 0.0.0.0
1.1.1.85 3-isopropylmalate dehydrogenase


D-erythro-3-Methylmalate + NAD+ <=> 2-oxobutyrate + CO2 + NADH 1.1.1
1.1.1.85 3-isopropylmalate dehydrogenase


citraconate + H2O <=> D-erythro-3-Methylmalate 4.2.1.35 (R)-2-methylmalate dehydratase

(R)-citramalate <=> citraconate + H2O 4.2.1.31 maleate hydratase
4.2.1.35 (R)-2-methylmalate dehydratase


(S)-2-Hydroxy-2-methyl-3-oxobutanoate <=> 3-hydroxy-3-methyl-2-oxobutanoic_acid 1.1.1.86 ketol-acid reductoisomerase (NADP+)
5.4.99.3 2-acetolactate mutase


L-valine + H2O + NAD+ <=> 2-oxoisovalerate + NH3 + NADH + H+ 1.4.1.1 alanine dehydrogenase
1.4.1.2 glutamate dehydrogenase
1.4.1.20 phenylalanine dehydrogenase
1.4.1.23 valine dehydrogenase (NAD+)
1.4.1.5 L-amino-acid dehydrogenase
1.4.1.8 valine dehydrogenase (NADP+)
1.4.1.9 leucine dehydrogenase







acetyl-CoA + pyruvate + H2O <=> H+ + (R)-citramalate + CoA 2.3.1.182 (R)-citramalate synthase
2.3.3.13 2-isopropylmalate synthase


H+ + pyruvate + 2-oxobutyrate <=> (S)-2-hydroxy-2-ethyl-3-oxobutanoate + CO2 2.2.1.6 acetolactate synthase

2R,3R-2,3-dihydroxy-3-methylpentanoate + NADP+ <=> (R)-3-hydroxy-3-methyl-2-oxopentanoate + NADPH + H+ 1.1.1.86 ketol-acid reductoisomerase (NADP+)

H+ + pyruvate <=> (S)-2-Hydroxy-2-methyl-3-oxobutanoate + CO2 2.2.1.6 acetolactate synthase
4.1.1.1 pyruvate decarboxylase


2R,3R-2,3-dihydroxy-3-methylpentanoate <=> (S)-3-methyl-2-oxopentanoate + H2O 4.2.1.9 dihydroxy-acid dehydratase

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